STRATEGIC OPTION OF MOBILE PHONE OPERATOR ORANGE UNDER CRISIS CONDITIONS

The current financial and economic crisis has affected many sectors, but companies have tried to implement marketing strategies adapted to the context. Telecommunication field was affected to a lesser extent, given the customers’ need for communication, need that doesn’t go away, but that can be achieved through orientation to other service providers. As a research methodology, we used qualitative methods and empirical data on the evolution of Orange mobile operator under crisis conditions, and compared with other operators. The main advantage of these methods is the adaptation to existing resources and external identifying limitations. The objective of this study is to understand how to manage effectively a crisis by applying different marketing strategies adapted to the changing customer needs.telecommunications; strategy; crisis; opportunity;option

A Community-Based Sensor Network for Monitoring the Air Quality in Urban Romania

Air quality, especially particulate matter pollution levels in urban areas, is an essential academic and social topic due to its association with health issues and climate change. In Romania, increasing awareness of urban communities and the availability of low-cost sensors has led to the development of an independent monitoring network currently distributed in over 194 cities and towns. The uRADMonitor((R)) network consists of 630 sensors measuring PM10 and PM2.5 concentration levels. The spatial distribution of the sensors complements the national air quality network with sensors in residential areas, intense traffic zones, and industrial areas. The data are available through a user-friendly web-based platform from uRADMonitor((R)). Based on data collected in 2021, we present an analysis of PM10 pollution levels in Romania's five most populated urban areas by employing five annual statistical indicators recommended by the European Environmental Agency. For the case of Timis,oara, we also compare the data measured by independent sensors with those from the national monitoring network. The results highlight the usefulness of our community-based network as it complements the national one

Classification of cancer cell lines using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and statistical analysis

Over the past decade, matrix-assisted laser desorption/ionization time‑of‑flight mass spectrometry (MALDI‑TOF MS) has been established as a valuable platform for microbial identification, and it is also frequently applied in biology and clinical studies to identify new markers expressed in pathological conditions. The aim of the present study was to assess the potential of using this approach for the classification of cancer cell lines as a quantifiable method for the proteomic profiling of cellular organelles. Intact protein extracts isolated from different tumor cell lines (human and murine) were analyzed using MALDI‑TOF MS and the obtained mass lists were processed using principle component analysis (PCA) within Bruker Biotyper® software. Furthermore, reference spectra were created for each cell line and were used for classification. Based on the intact protein profiles, we were able to differentiate and classify six cancer cell lines: two murine melanoma (B16‑F0 and B164A5), one human melanoma (A375), two human breast carcinoma (MCF7 and MDA‑MB‑231) and one human liver carcinoma (HepG2). The cell lines were classified according to cancer type and the species they originated from, as well as by their metastatic potential, offering the possibility to differentiate non‑invasive from invasive cells. The obtained results pave the way for developing a broad‑based strategy for the identification and classification of cancer cell

HOLMES, an experiment for a direct measurement of neutrino mass

The neutrino mass is a fundamental parameter of the Standard Model and the measurement of its value is one of the most compelling issues in particle physics. HOLMES is an experiment set up at the University of Milano-Bicocca aiming at performing a direct measurement of the neutrino mass from the Electron Capture (EC) decay of 163Ho. HOLMES will use low-temperature calorimeters, avoiding the typical systematics of spectrometers arising from the use of any external source, in order to precisely measure the energy of the electrons emitted in the EC decay. In this contribution we outline the steps which will lead to the HOLMES measurement of the neutrino mass

Internet of things

Manual of Digital Earth / Editors: Huadong Guo, Michael F. Goodchild, Alessandro Annoni .- Springer, 2020 .- ISBN: 978-981-32-9915-3Digital Earth was born with the aim of replicating the real world within the digital world. Many efforts have been made to observe and sense the Earth, both from space (remote sensing) and by using in situ sensors. Focusing on the latter, advances in Digital Earth have established vital bridges to exploit these sensors and their networks by taking location as a key element. The current era of connectivity envisions that everything is connected to everything. The concept of the Internet of Things(IoT)emergedasaholisticproposaltoenableanecosystemofvaried,heterogeneous networked objects and devices to speak to and interact with each other. To make the IoT ecosystem a reality, it is necessary to understand the electronic components, communication protocols, real-time analysis techniques, and the location of the objects and devices. The IoT ecosystem and the Digital Earth (DE) jointly form interrelated infrastructures for addressing today’s pressing issues and complex challenges. In this chapter, we explore the synergies and frictions in establishing an efficient and permanent collaboration between the two infrastructures, in order to adequately address multidisciplinary and increasingly complex real-world problems. Although there are still some pending issues, the identified synergies generate optimism for a true collaboration between the Internet of Things and the Digital Earth

A whole-genome assembly of the domestic cow, Bos taurus

Background: The genome of the domestic cow, Bos taurus, was sequenced using a mixture of hierarchical and whole-genome shotgun sequencing methods. Results: We have assembled the 35 million sequence reads and applied a variety of assembly improvement techniques, creating an assembly of 2.86 billion base pairs that has multiple improvements over previous assemblies: it is more complete, covering more of the genome; thousands of gaps have been closed; many erroneous inversions, deletions, and translocations have been corrected; and thousands of single-nucleotide errors have been corrected. Our evaluation using independent metrics demonstrates that the resulting assembly is substantially more accurate and complete than alternative versions. Conclusions: By using independent mapping data and conserved synteny between the cow and human genomes, we were able to construct an assembly with excellent large-scale contiguity in which a large majority (approximately 91%) of the genome has been placed onto the 30 B. taurus chromosomes. We constructed a new cow-human synteny map that expands upon previous maps. We also identified for the first time a portion of the B. taurus Y chromosome. © 2009 Zimin et al.; licensee BioMed Central Ltd

First array of enriched Zn82^{82}Se bolometers to search for double beta decay

The R&D activity performed during the last years proved the potential of ZnSe scintillating bolometers to the search for neutrino-less double beta decay, motivating the realization of the first large-mass experiment based on this technology: CUPID-0. The isotopic enrichment in $^{82}$Se, the Zn$^{82}$Se crystals growth, as well as the light detectors production have been accomplished, and the experiment is now in construction at Laboratori Nazionali del Gran Sasso (Italy). In this paper we present the results obtained testing the first three Zn$^{82}$Se crystals operated as scintillating bolometers, and we prove that their performance in terms of energy resolution, background rejection capability and intrinsic radio-purity complies with the requirements of CUPID-0

Развитиe преподавания семейной медицины в университетских программах

Department of Family Medicine, Nicolae Testemitanu State Medical and Pharmaceutical University, Congresul III al Medicilor de Familie din Republica Moldova, 17–18 mai, 2012, Chişinău, Republica Moldova, Conferinţa Naţională „Maladii bronhoobstructive la copii”, consacrată profesorului universitar, doctor habilitat Victor Gheţeul, 27 aprilie, Chişinău, Republica MoldovaIntroducere. Tendinţele actuale ale Strategiei naţionale de modernizare a Sistemului Ocrotirii Sănătăţii din Republică prevăd dezvoltarea continuă a sectorului de asistenţă medicală primară în baza specialităţii Medicina de familie. Una din priorităţile fundamentale ale întregii ţări este integrarea europeană. Instruirea universitară în Medicina de familie a fost iniţiată în anul universitar 2007-2008 pentru studenţii anului VI, Facultatea de Medicină. Reactualizarea a demarat în 2009 şi a intrat în vigoare din ianuarie 2010. Astfel, programele educaţionale în domeniul medicinii de familie la etapa universitară şi postuniversitară trebuie să fie concordate cu cerinţele europene şi internaţionale. Ţinând cont de specificul şi variabilitatea regională a asistenţei medicale primare pentru ţările din Europa, autorii programului s-au condus de Agenda Educaţională EURACT, urmând competenţele de bază pentru instruire. Material şi metode Modificarea curriculei universitare la disciplina Medicina de familie a fost realizată după expertizarea ei de către colaboratorii catedrei Medicină de Familie a USMF „Nicolae Testemiţanu” împreună cu echipa de experţi-consultanţi în reforme educaţionale din România în cadrul proiectului „Îmbunătăţirea Conţinutului Cursurilor de Educaţie a Medicilor de Familie, Managerilor Instituţiilor de Medicina Primară şi a Asistenţilor Medicilor de Familie din Republica Moldova”, realizat de Programul de Servicii de Sănătate şi Asistenţă Socială prin intermediul Centrului pentru Politici şi Servicii de Sănătate din Bucureşti, România. Rezultate Astfel, pe parcursul anului 2009 au fost revizuite: Programul de instruire universitar la disciplina Medicină de familie pentru studenţii anului V, facultatea Medicină, şi anului VI, facultatea Sănătate Publică; Programul de instruire prin rezidenţiat pentru toţi trei ani, la Specializarea Medicină de familie; 3 module din cadrul programului de Educaţie Medicală Continuă pentru medicii de familie. Toate aceste programe au fost recenzate şi aprobate în cadrul şedinţei catedrei Medicină de familie, şedinţei Comisiei Metodice de Profil Boli interne, şedinţei Consiliului Metodic Central al USMF “Nicolae Testemiţanu” şi de către Ministrul Sănătăţii din Republica Moldova. În cadrul revizuirii curriculum-ului universitar s-a urmărit scopul de a adapta programul de instruire la cerinţele educaţionale europene. Obiectivele le-au constituit: formarea conceptului despre specialitatea Medicină de familie şi familiarizarea cu particularităţile activităţii medicului de familie. A fost redusă ponderea orelor teoretice în favoarea practicii în oficiul medicului de familie, solicitat de către studenţii instruiţi în anii precedenţi. Astfel, durata cursului a crescut de la 10 la 15 zile, raportul teorie/practică de la 30% şi 70% în anul 2007 la 10% şi 90% respectiv în anul 2011. Metodele moderne interactive de predare au fost alese pentru aplicare în cadrul programului: prelegere interactivă, lecţie practică cu utilizarea studiului de caz, jocuri pe roluri, lucru în grupuri mici, lucru individual, discuţie plenară, observare clinică, completarea documentaţiei medicale. Metodele de evaluare au fost divizate în două modalităţi: curentă şi finală. Anul de studii 2010-2011 a fost marcat de trecerea instruirii studenţilor în domeniul medicinii de familie de la anul VI la anul V de studii. De la începutul implementării programului universitar au fost instruiţi în total 2203 studenţi, dintre care 909 studenţi – conform curriculum-ului revizuit şi adaptat. Concluzii În cadrul revizuirii programului de studii universitar a fost atins scopul de a ajusta curriculum-ul existent la cerinţele Agendei Educaţionale EURACT. Este necesară revizuirea sistematică a programului de studii universitar, cel puţin o dată la 5 ani pentru menţinerea conţinutului acestora la nivelul practicilor contemporane internaţionale şi implementării metodelor eficiente de instruire a competenţelor profesionale în specialitate